Description
Anti-Camelid VHH Affinity Resin (JOT1020) is designed for the purification of Camelid VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified Camelid VHH single domain antibody is eluted with a high recovery rate.
Dosage options: 1mL / 5mL / 10mL
Table 1 lists the main characteristics of Anti-Camelid VHH Affinity Resin.
Table 1. Characteristics of Anti-Camelid VHH Affinity Resin
| Product content | 50% settled resin in TBS with 0.02% sodium azide |
| Matrix | 4% cross-linked agarose |
| Average bead size | 90 μm |
| Ligand | Rabbit Anti-Camelid VHH Antibody |
| Binding capacity | Approximately 2-4 mg Camelid VHH protein per mL settled resin (depending on amino acid sequence of different Camelid VHHs) |
| Storage and stability | Store at 2-8 °C for up to 12 months. Do not freeze the resin. |
| Resin reuse | When stored at 2 – 8° C, the resin can be recycled at least 5 times with no loss of binding capability. The resin can be reused up to 10 times with minimum loss of binding capability. |
| Elution method | Acid buffer elution (pH 2.5) |
| Reagents compatibility | Compatible with commonly used bacterial lysis reagents. For reagents compatible with purification, see tables 2 and 3 in the manual. |
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